College of Pharmacy, Second Military Medical University, Shanghai 200433.
The inhibitory effect of gelsemium alkaloids exstract (GAA) on HepG2 cells in vitro were studied by crystal violet dyeing method. The morphological change of HepG2 cells were observed with optical microscope. The alterations of cell cycle induced by GAA were analyzed with flow cytometry. The results showed that HepG2 cells exposed to GAA 10 micrograms/ml was inhibited significantly (P < 0.05). The inhibitory effect appeared in a dose- and time-dependent manner. HepG2 cells showed nuclear chromosome segmentation and condensation after GAA treatment. There emerged obvious Sub-G1 peak in the DNA histogram of HepG2 cells. GAA has a significant inhibition on HepG2 cells in vitro. The mechanism of antitumor action may be related to their apoptosis inducing activity.
PMID: 11715196 [PubMed - indexed for MEDLINE]